Sequences

Oligos

Name	Sequence (5'-->3')	Notes
CM-FWD-S2-i5	AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTCGTCGGCAGCGTC	The N8 sequence is where the i5 Illumina index should be placed
CM-REV-S2-i7	CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTCTCGTGGGCTCGG	The N8 sequence is where the i7 Illumina index should be placed

See below for csvs containing forward and reverse primers that can be used in stage 2 of barcode amplification reactions. These were generated by using index sequences provided in NEBNext^® Multiplex Oligos for Illumina^® (E6448S).

• ClonMapper Sequences
• ClonMapper S2 Sequences
• ClonMapper S2 Pairings

Plasmids

Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Addgene #137993

Recall-miniCMV-sfGFP

Addgene #137995